Abstract: Objective: To search for tumor suppressor genes associated with colorectal cancer by looking for high-frequency loss of heterozygosity (LOH) sites on the long arm of chromosome 4. Methods: Polymerase chain reaction (PCR) was performed with fluorescently labeled microsatellite primers and 83 tumor and normal tissues of colorectal cancer. PCR products were analyzed by electrophoresis on an ABI 377 DNA sequencer, and scanned and analyzed for LOH using Genescan 3.7 and Genotype 3.7 software. The LOH results were compared with clinical and pathological factors by χ2 test. Results: The average LOH rate of the long arm of chromosome 4 (4q) was 28.56%, and two obvious high-frequency LOH intervals were found, one between D4S3000 and D4S2915 sites (4q12-21.1); the other between D4S407 and D4S2939 sites (4q25-31.1). At the D4S3018 site, the LOH rate of tumors with a diameter of >5 cm was significantly higher than that of tumors with a diameter of >5 cm.
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